High Throughput INSTRUMENTATION
BioTrove: a revolutionary plate format that enables the performance of PCR and Real Time PCR in 33nL reactions.
Each OpenArrayTM contains 48 sub arrays of 64 thru-holes. Each hole holds a single reaction.
Gene Expression: reactions are performed using SYBR Green® chemistry. Each sample to be analysed is loaded on an individual sub array by the BioTrove AutoLoader instrument. For each sub array, up to 56 targets can be analysed.
In a single run, the BioTrove NT cycler can amplify and analyse three OpenArrayTMs, allowing 144 samples or 8064 QPCR reactions to be performed simultaneously.
For more information, please contact us.
Each OpenArrayTM contains 48 sub arrays of 64 thru-holes. Each hole holds a single reaction.
Gene Expression: reactions are performed using SYBR Green® chemistry. Each sample to be analysed is loaded on an individual sub array by the BioTrove AutoLoader instrument. For each sub array, up to 56 targets can be analysed.
In a single run, the BioTrove NT cycler can amplify and analyse three OpenArrayTMs, allowing 144 samples or 8064 QPCR reactions to be performed simultaneously.
For more information, please contact us.
OpenArrayTM plates
One OpenArray contains as many reactions as 8 x 384 wells plates
Standard curve 101 to 106 copies
Uniformity run on 100 copies
3000 replicates
3000 replicates
Pathogen detection & Genotyping: reactions are performed using Taqman™ assays. Each sample to be analysed can be loaded on 1 sub array (64 reactions), or half a sub array (32 reactions) or a third of a sub array (16 reactions) allowing the analysis of up to 144 samples per OpenArrayTM and 432 samples per RUN directly on the cycler.
PCR can be performed off-line on a regular PCR instrument when higher throughput is required. In this mode, up to 32 OpenArrays or 198,000 genotypes (the equivalent of 256 x 384 well plates), can be processed in one day, by a single technician.
PCR can be performed off-line on a regular PCR instrument when higher throughput is required. In this mode, up to 32 OpenArrays or 198,000 genotypes (the equivalent of 256 x 384 well plates), can be processed in one day, by a single technician.
Applicaton notes with examples are available.
For more information, please contact us.
For more information, please contact us.
SNP analysis
Methylation Specific PCR
64 targets on 48 samples
16 targets on 144 samples