System Biosciences TransDux MAX

Building on SBI’s popular TransDux™ virus transduction reagent (Cat# LV850A-1), TransDux MAX (Cat# LV860A-1) further increases transduction efficiencies by 4-fold, for better, more reliable lentiviral gene expression across a wide range of cell types and with any lentivector.

TransDux MAX comes in an easy-to-use, two component format (TransDux and MAX Enhancer) good for 100 transduction reactions. Simply mix both reagents into conditioned media from target cells, and infect with your lentivirus of choice for a higher level of transduction efficiency.

 

TransDux MAX delivers high transduction efficiencies across a broad range of cell types. Transduction of SBI’s LV605 pre-packaged virus [MSCV-GFP-T2A-RFP] into various cell lines (HT1080, HepG2, and Jurkat T-cells). Cells were imaged for RFP expression 72 hours post-infection.

Efficient—up to 8-fold greater transduction efficiency than polybrene, and 4-fold greater efficiency than standard TransDux reagent as measured by qPCR
• Easy-to-use—kit format requires minimal hands-on time (
• Non-toxic—no need to change out media after infection
• Versatile—works with all types of packaged lentivirus
• Flexible—enhances transduction efficiency in a wide variety of cell lines
• Broadly compatible—does not interfere with downstream gene expression or functional assays

TransDux MAX delivers even higher transduction efficiencies than the original TransDux reagent. SBI’s BLIV713 pre-packaged lentivirus [CMV-Luciferase-EF1a-copGFP-T2A-Puro] was transduced into HT1080 cells using either TransDux MAX, TransDux, or Polybrene. Cells were imaged for GFP expression 72 hours post-infection.

 

        qPCR-based quantitation of virus infectivity