Integrated Sciences is excited to introduce SBI's all-new, exclusive CLOuD9 Gene Expression Regulation System that puts you in charge of chromatin loop formation.

Introduced in a recent Nature Communications paper1, the inducible and reversible CLOuD9 System gives you the control you need over chromatin loop formation so you can explore the dynamic effects of chromatin restructuring on gene expression.
Combining the ease-of-targeting possible through CRISPR/Cas9 with an inducible and reversible protein-protein dimerization pair, CLOuD9 takes your gene regulation research to the next level.

Morgan, SL, et al. Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping.Nat Commun. 2017. Jul 13; 8:15993. PMCID: PMC5511349

CLOuD9 for Chromatin Loop Reorganization using CRISPR-dCas9:

  1. Innovative—leverage the simplicity of CRISPR/Cas9 targeting—free of DNA cleavage activity—for inducible and reversible chromatin loop formation anywhere in the genome
  2. Well-validated—chromatin loop formation and the resulting changes in gene expression across multiple loci and cell lines is amply demonstrated in Morgan, et al,1
  3. Broadly applicable—great for studying the role of chromatin in the control of gene expression in a range of areas, including:
    a) Developmental biology
    b) Cancer biology
    c) Chromatin structural biology
    d) Chromatin-based therapeutic strategies
    e) Unique—the innovative CLOuD9 System is only sold by SBI

The CLOuD9 Gene Expression Regulation Kit comes with CLOuD9 Inducer Reagent and the two lentivector plasmids needed for expressing CLOuD9’s CRISPR/Cas9 System components (Figure 1):

One lentivector plasmid expressing a null Staphylococcus aureus Cas9 protein fused to one of the protein dimerization partners—the ABI protein from the plant abscisic acid (ABA) signaling pathway
A second lentivector plasmid expressing a null S. pyogenes Cas9 protein fused to the other protein dimerization partner—the PYL1 protein from the plant ABA signaling pathway

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Figure 1. CLOuD9 constructs

 Read the Nature Communications Article here
 

                            Order here

 

1. Morgan, SL, et al. Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping. Nat Commun. 2017. Jul 13; 8:15993. PMCID: PMC5511349.