Integrated Sciences is pleased to offer 30% off list price** selected qPCR and qRT-PCR bioreagents for all orders placed through the Integrated Sciences website.

**Promotion is limited to online web ordering only. Special offer ends 30th June 2017

AffinityScript cDNA Synthesis kit

  • Robust cDNA synthesis across a broad range of temperatures from 37–55°C
  • Linear detection from 3pg to 3µg total RNA
  • Streamlined protocol produces cDNA in 15 minutes and an overall protocol time of just 25 minutes
  • High affinity for primer-template complexes promotes optimal cDNA yield
  • Can synthesize long cDNA targets, up to 12 kb, from small amounts of input RNA
  • Able to reverse transcribe through RNA secondary structures
  • Strict enzyme purity specifications ensure the absence of contaminating RNase and exonuclease activities
Eligible Products Catalogue Codes
AffinityScript QPCR cDNA Synthesis Kit 600559
AffinityScript Multiple Temperature Reverse Transcriptase 600105
600107
600109


Brilliant III SYBR and Probe qPCR Master Mix

  • Brilliant III qPCR Master Mix maintains amplification efficiency, R2, dynamic range and detection sensitivity all while shortening the total cycling time by ~60% compared to standard cycling conditions
  • Enhanced rapid hot start capability saves time and reduces primer-dimer formation
  • Greater resistance to common qPCR inhibitors (i.e. whole blood or NaCl)
  • Convenient pre-blended formulations compatible with any SYBR Green dye detection or sequence-specific probe detection chemistry.
  • Available with low or high ROX concentrations
Eligible Products Catalogue Codes
Brilliant III Ultra-Fast qPCR Master Mix 600880
600881
Brilliant III Ultra-Fast Probe ROX qPCR Master Mix 600888
600890
600898
600899
Brilliant III Ultra-Fast qRT-PCR Master Mix 600884
600885
Brilliant III Ultra-Fast SYBR Green qPCR Master Mix 600882
600883
Brilliant III Ultra-Fast SYBR Green ROX qPCR Master Mix 600889
600904
600892
600903
Brilliant III Ultra-Fast SYBR Green qRT-PCR Master Mix 600886
600887

 

Brilliant II SYBR and Probe qPCR Master Mix

  • Earlier threshold cycle (Ct) detection at high and low template concentrations
  • Improved reaction efficiency and replicate reproducibility across the entire range of template concentrations
Eligible Products Catalogue Codes
Brilliant II qPCR Master Mix 600804
600815
Brilliant II qPCR ROX Master Mix 600805
600806
Brilliant II qRT-PCR 1-Step Master Mix 600809
600818
Brilliant II qRT-PCR ROX 1-Step Master Mix 600837
600838
Brilliant II qRT-PCR AffinityScript 2-Step Master Mix 600827
Brilliant II SYBR Green qPCR Master Mix 600828
600831
Brilliant II SYBR Green ROX qPCR Master Mix 600829
600830
Brilliant II SYBR Green qRT-PCR 1-Step Master Mix 600825
600826
Brilliant II qRT-PCR SYBR Green ROX Master Mix 600835
600836
Brilliant II SYBR Green qRT-PCR AffinityScript 2-Step Master Mix 600834

 

Brilliant Multiplex qPCR Master Mix

  • The Brilliant Multiplex qPCR Master Mix allows you to amplify up to four targets in a single real-time PCR Reaction
  • Allows successful multiplexing without concern for bias due to the abundance level. Importantly, the sensitivity remains equivalent to that seen in singleplex.
Eligible Products Catalogue Codes
Brilliant Multiplex qPCR Master Mix  600553

 

Don't know which qPCR reagents to use? Use Agilent Technologies qPCR Decision Tree to find out which qPCR solution best fits your application.