Zymo's Quick-DNA™ Fecal/Soil Microbe 96 Magbead Kits

The Quick-DNA™ Fecal/Soil Microbe 96 Magbead Kit is designed for the simple and rapid isolation of inhibitor-free, PCR-quality DNA from a variety of fecal (including humans, birds, rats, mice, cattle, etc.) and soil (including clay, sandy, silty, peaty, chalky, and loamy soils) samples. The procedure is easy and can be completed in as little as 90 minutes per 96 samples. Fecal (≤100 mg) and soil (≤200 mg) samples are added directly to a ZR BashingBead™ Lysis Rack (0.1 & 0.5 mm) and rapidly and efficiently lysed by bead beating, without the use of organic denaturants or proteinases. The DNA is then isolated and purified using our Zymo MagBinding Bead Technology, which features our Inhibitor Removal Technology built into the buffer system. Simply bind, wash, and elute with the Quick-DNA™ Fecal/Soil Microbe 96 Kit to recover DNA that is ideal for downstream molecular-based applications including PCR, arrays, genotyping, etc.

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The Quick-DNA™ Fecal/Soil Microbe 96 Magbead kit produces linear recovery of DNA for sensitive applications, detecting pathogenic organisms such as E. coli and H. pylori in assays with up to a 1000x dilution factor. A dilution series of stool spiked with 1x10^6 E. coli cells and H. Pylori
infected stool was extracted using the Quick
-DNA™ Fecal/Soil Microbe 96 Magbead Kit, showing effective purification and qPCR amplification, even at 1000:1 dilution. Healthy stool sample controls
did not amplify pathogenic organisms compared to the spiked stools. N=8.
  The Quick DNA Fecal/Soil Microbe 96 Magbead Kit provides inhibitor - free DNA even when challenged with extremely inhibitor rich samples. Real - time PCR was used to evaluate eluates recovered using the QuickDNA Fecal/Soil Microbe 96 Magbead Kit or Supplier A. Reaction volumes consisted of
either 10% or 35% of the eluate from each kit to detect the presence of PCR inhibitors. Each reaction contained 10 ng of
Brettanomyces DNA. Delayed and/or no amplification indicates PCR inhibition from inefficient inhibitor removal. N=8

The Quick-DNA™ Fecal/Soil Microbe 96 Magbead Kit provides superior yields when compared to Supplier A. 80 mg of feces was processed using each kit according to the manufactures’ recommended protocol. DNA was eluted using 100 μl DNA Elution Buffer. 6 μl of each sample was analyzed in a 1.0% (w/v) agarose/ethidium bromide gel. Samples were processed in triplicate. L is a 1Kb ladder.

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